Services

Facility and Instrumentation

• Field strengths at 400, 500, 600, and 700MHz – 1H , 19F, and X nuclei from 15N – 31P
• Most systems have cryogenic NMR probes for maximum sensitivity
• Several systems are highly automated for routine NMR
• 800 and 900 MHz available for scientifically justified samples
• Modern systems, highly experienced and dedicated staff

Major Service Categories

• Protein NMR projects and data collection for metabolomics studies
• Fragment-based screening and lead discovery
• NMR of natural products for structure elucidation (mass limited samples)
• Medicinal chemistry support
• Metabolomics (urine, blood, other bio-fluids)
• Routine NMR for organic synthesis

Details of services offered to EQNMR users:

Synthetic and medicinal chemistry support

• Walkup environment to maximize throughput and convenience for routine samples
• Full complement of optimized one and two-dimensional experiments
• Structure verification services available via our corporate partners

NMR for structure elucidation of natural products and mass limited samples

• Full complement of 1D and 2D NMR techniques for challenging molecules
• Cryogenic probes are available to provide absolute highest sensitivity
• 5, 3, and 1.7mm tubes can be accommodated to optimize sensitivity and expt. time
• State-of-the-art Non-uniform sampling for heteronuclear experiments

Fragment-based screening and lead discovery

• Well developed ligand and protein-detected fragment screening platform
• Fragment libraries (proton and fluorine) available for screening
• 1H and 19F detection for assessment and characterization of ligand binding
• Liquid handler for sample prep and high capacity auto-sampler for data collection

Property-based ligand binding studies

• Assessment and characterization of non-fragment ligands that bind macromolecules
• Rapid identification of ligand aggregation or solubility issues
• NMR assessment of hits from HTS or other screening methods

Protein NMR services

• Data collection and processing in preparation for resonance assignments for small to medium sized proteins
• All protein NMR systems are modern and equipped with cryogenic probes
• Advice on sample preparation for NMR studies

Metabolomics

• Data collection for any type of bio-fluid or soluble cell or tissue extract samples
• 1H and 31P NMR capability

Specialty projects can be considered – contact Greg Heffron (gregory_heffronhms.harvard.edu (gregory_heffron[at]hms[dot]harvard[dot]edu))

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